莊盟生物

當前位置:首頁 - 產品中心 - 分子克隆相關產品

產品名稱:pRS316(ZK958)

貨號 規格 價格 訂購數量 是否現貨
ZK958 1ug×1支 780 - + 有貨

■ 基本信息

啟動子:URA3,T7,T3

復制子:pUC ori,FI ori

質粒分類:酵母系列,釀酒酵母表達載體

質粒大小:4887bp

原核抗性:Amp

篩選標記:URA3

克隆菌株:DH5α

培養條件:37℃,有氧 LB

表達宿主:酵母細胞

誘導方式:半乳糖誘導

5'測序引物:T7:TAATACGACTCACTATAGGG

3'測序引物:M13R:CAGGAAACAGCTATGACC


質粒簡介

This is one of a series of pBluescriptbased YCtype (centromeric) yeast shuttle vectors (ATCC 7714277145) differing in the yeast selectable marker gene. It encodes the beta galactosidase alpha peptide for blue white color detection of inserts and also has the T7 and T3 promoters for in vitro RNA synthesis and priming sites for sequencing. It is useful in plasmid shuffling experiments. It was constructed by inserting a 1.112 kb fragment containing the URA3 gene into the NdeI site of the pRSS56 vector and also inserting a cassette containing CEN6 and the ARS associated with histone 4 (ARSH4) into the AatII site of the same vector. All ends were blunted. The pRSS56 vector was constructed by ligating a PvuI fragment (bp 4982412) of pBluescript KS+ and the fragment from the unique NdeI and AatII sites between bla and f1 origin of pBS(+). The order of the major features in this plasmid is: URA3  f1 ori (NaeI)  T7 promoter  lacZ/MCS  T3 promoter pMB1 ori  bla  CEN6  ARSH4.


載體質粒圖譜和多克隆位點信息




夜夜澡 人人碰 人人看,夜夜橾天天橾在线视频,天天夜夜日日高清在线-首页