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產品名稱:pBI221-EGFP(ZK855)

貨號 規格 價格 訂購數量 是否現貨
ZK855 1ug×1支 780 - + 有貨
質粒分類
主要功能次級功能用途抗性
植物系列質粒
植物表達質粒
pBI系列質粒

Amp


■ 基本信息

啟動子:CaMV 35S ,Lac

復制子:ori

終止子:NOS

質粒分類:植物系列,蛋白過表達載體

質粒大小:4530bp

原核抗性:Amp

克隆菌株:DH5α

培養條件:37℃,有氧 LB

5'測序引物:35S:GACGCACAATCCCACTATCC

3'測序引物:根據序列設計引物


■ 質粒簡介

Most studies related to determining the expression profile of genes and specific promoters used histochemical localization of the reporter gene, gusA. While the histochemical method for visualizing gusA expression suffers from several limitations in the determination of gene expression and location, especially in the tissues with high background acitivty. To solve this problem, a transient expession vector pBI221-RFP/GFP, was constructed using GFP and RFP as double fluorescent marker genes. This vector used CaMV 35S promoter to drive GFP and determine the transforming efficiency. It analyzed expression profile of the target gene and promoter through the RFP activities of the tranformed tissues. Through the specific promoter AGPL1 from watermelon and E8 promoter from tomato, it is resistible to use this vector to study the expression patterns of promoters. Results indicated that the pBI221-RFP/GFP is a very efficient transient expression vector that can be verify the functions of the genes and promoters.


■ 質粒圖譜


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